WEIGHT: 49 kg
Sex services: Massage Thai, Blow ride, Oral, Travel Companion, Dinner Dates
This expansion has created conditions that facilitate the generation of new recombinant strains. Based on pol nucleotide sequences, a total of 19 strains 1. Co-circulation of multiple lineages of HIV-1 strains had led to the inevitable emergence of various forms of inter-genotype recombinants and of novel circulating recombinant forms CRFs. All the methods involving human subjects were carried out in accordance with relevant approved guidelines and regulation.
All study subjects provided informed consent regarding the provision of blood samples and HIV-genotype analyses. The genotypes were determined based on the phylogenetic analyses to partial pol gene HXB2: — nt.
The percentage of case number in each subtype or CRFs are shown in parentheses. The pol gene sequences HXB2 — nt were amplified using a previously published method 9. Amplicons were sequenced by Beijing Huada Scientific Corporation Beijing using internal walking primers.
Alignment and manual editing were performed using Clustal X software Version 2. Phylogenetic analyses were performed using the neighbor-joining method based on the Kimura 2-parameter distance matrix and a transition-to-transversion ratio of 2.
Tree topology was tested by bootstrap analysis with 1, replicates. HIV-1 recombinant analysis was carried out using Simplot Version 3. Bayesian Markov chain Monte Carlo MCMC inference under the relaxed lognormal molecular clock was selected as a reliable mode for this analysis The MCMC chains were run 20 million times and sampled every steps.